Job Description

The Myc oncoprotein is dysregulated in most human cancers and is a key regulator of gene transcription, binding to ~10% of the human genome to drive biological activities associated with the hallmarks of cancer. Successfully targeting MYC would represent a breakthrough in cancer therapy, but due to the intrinsically disordered nature of the MYC protein it has evaded classic drug development approaches for several decades. Our lab has recently had a breakthrough and identified several proteins that interact with MYC. These data provide an unprecedented opportunity to i) better understand MYC regulation and function as a potent cancer driver, and ii) develop anti-MYC activity therapies by blocking critical MYC-protein interactions. This novel line of research has enormous potential to radically advance new knowledge and make a lasting impact to patients and their families.

We are searching for a highly motivated Post-Doctoral Researcher with a PhD in a related discipline, such molecular biology or biochemistry. This project will consist of validating and characterizing novel MYC-protein interactions at the cellular and tumor levels, with a focus on proteins that are essential to MYC oncogenic function. The successful candidate will be working at the Princess Margaret Cancer Centre and will benefit from a highly collaborative and interdisciplinary research team.

Duties:

• Conduct protein-protein interaction assays, such as proximity ligation assay, co-immunoprecipitation
• Analyze protein-DNA interactions using CUT&RUN or Chromatin Immunoprecipitation followed by sequencing or qPCR
• Perform analyses of gene expression including RT-qPCR, RNA-seq, EU incorporation
• Assay MYC-protein complexes regulating gene transcription and oncogenesis by a variety of methods
• Have strong computational skills to enable genomic analyses and data integration
• Work with databases that enable our research such as Encode and DepMap
• Understand the state-of-the-art at both the methodological and biological levels 
• Report results both in writing and orally to individuals as well as collaborative groups

Qualitifications:

• PhD in molecular biology, biochemistry or related discipline
• Expertise in the area of gene transcription and protein-protein interaction
• Comprehensive experience in using and developing tumor models based in tissue culture and mice
• Work well independently as well as with others as part of a team
• Excellent problem recognition and problem-solving
• Excellent verbal and written communication skills
• Excellent interpersonal skills
• Excellent organizational and time management skills
• Ability to set priorities and work independently with accuracy in a fast-paced multitasking environment

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